Margaret Urquhart studied double bass and violone with Anthony Woodrow and viola da gamba with Anneke Pols at the Royal Conservatory in The Hague. After completing her studies she became a member of many notable baroque ensembles, including La Petite Bande (Sigiswald Kuijken), Amsterdam Baroque Orchestra (Ton Koopman), Leonhardt Consort (Gustav Leonhardt), and Collegium Vocale Gent (Philippe Herreweghe).

Since 1986 she has been a member of the Orchestra of the Eighteenth Century and has been active as a chamber music performer taking part in many radio, TV, and CD recordings.
 
She has taught baroque double bass and violone at The Hague Conservatory since 1989, and at the Conservatorium van Amsterdam since 1999. In addition to her two teaching post she also teaches the double bass and violone at the University of Salamanca, and coaches at the European Baroque Orchestra training courses.

She performs and teaches on the 16-foot double bass, the Viennese double bass, both 8-foot and 16-foot violone in various tunings.